Overview

  • Product name
  • Description
    Rabbit polyclonal to Sp8
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Mouse Sp8.

    (Peptide available as ab90836.)

  • Positive control
    • This antibody gave a positive signal in 46C whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab73494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 48 kDa).

Target

  • Function
    Transcription factor which plays a key role in limb development. Positively regulates FGF8 expression in the apical ectodermal ridge (AER) and contributes to limb outgrowth in embryos.
  • Sequence similarities
    Belongs to the Sp1 C2H2-type zinc-finger protein family.
    Contains 3 C2H2-type zinc fingers.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • BTD antibody
    • Buttonhead antibody
    • Homolog of Btd antibody
    • OTTHUMP00000120017 antibody
    • Sp 8 antibody
    • SP8 antibody
    • Sp8 transcription factor antibody
    • SP8_HUMAN antibody
    • Specificity protein 8 antibody
    • Trans acting transcription factor 8 antibody
    • Transcription factor Sp8 antibody
    see all

Images

  • Anti-Sp8 antibody (ab73494) at 1 µg/ml + 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate (ab27205) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 48 kDa
    Observed band size : 46 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 23 kDa,56 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes

References

ab73494 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab73494.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up