Overview

  • Product name
  • Description
    Rabbit polyclonal to SPACA3
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: TNNGIFQINS RRWCSNLTPN VPNVCRMYCS DLLNPNLKDT VICAMKITQE PQGLGYWEAW RHHCQGKDLT EWVDGCDF, corresponding to amino acids 138-215 of Human SPACA3.

  • Positive control
    • Human testis tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab121768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/2500 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • Function
    Sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. It could be a potential receptor for the egg oligosaccharide residue N-acetylglucosamine, which is present in the extracellular matrix over the egg plasma membrane. The processed form has no detectable bacteriolytic activity in vitro.
  • Tissue specificity
    The processed form is expressed in sperm (at protein level). Expressed in testis, epididymis and placenta.
  • Sequence similarities
    Belongs to the glycosyl hydrolase 22 family.
  • Post-translational
    modifications
    The processed form derives from the membrane form by proteolytic processing.
  • Cellular localization
    Secreted and Cytoplasmic vesicle > secretory vesicle > acrosome membrane. Anterior acrosome in non-capacitated spermatozoa and retained in the equatorial segment and in the luminal face of both the inner and outer acrosomal membranes following capacitation and the acrosome reaction.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1700025M08Rik antibody
    • ALLP17 antibody
    • Cancer/testis antigen 54 antibody
    • CT54 antibody
    • LYC3 antibody
    • Lysozyme like sperm specific secretory protein ALLP17 antibody
    • Lysozyme-like acrosomal sperm-specific secretory protein ALLP-17 antibody
    • Lysozyme-like protein 3 antibody
    • LYZL3 antibody
    • processed form antibody
    • SACA3_HUMAN antibody
    • SLLP1 antibody
    • SPACA3 antibody
    • Sperm acrosome associated 3 antibody
    • Sperm acrosome membrane-associated protein 3 antibody
    • Sperm lysozyme-like protein 1 antibody
    • Sperm protein reactive with antisperm antibodies antibody
    • Sperm protein reactive with ASA antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121768 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121768 at a 1/2500 dilution staining SPACA3 in paraffin embedded Human testis tissue by Immunohistochemistry.
  • Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
    Lane 2: Negative control (vector only transfected HEK293T lysate)
    Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells. Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
    Lane 2: Negative control (vector only transfected HEK293T lysate)
    Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.

References

ab121768 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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