Overview

  • Product name
  • Description
    Rabbit polyclonal to SPACA4
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: MHCGDDEDCF TGHGVAPGTG PVINKGCLRA TSCGLEEPVS YRGVTYSLTT NCCTGRLCNR APSSQTVGAT TSLALGLGML LPPR, corresponding to amino acids 39-122 of Human SPACA4.

  • Positive control
    • Human testis tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration.

Target

  • Function
    Sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization.
  • Tissue specificity
    Testis specific. Expressed in spermatozoa.
  • Sequence similarities
    Contains 1 UPAR/Ly6 domain.
  • Cellular localization
    Cell membrane. Cytoplasmic vesicle > secretory vesicle > acrosome. Expressed in acrosomal matrix and outer and inner acrosomal membranes.
  • Information by UniProt
  • Database links
  • Alternative names
    • SACA4_HUMAN antibody
    • SAMP14 antibody
    • SPACA4 antibody
    • sperm acrosomal membrane protein 14 antibody
    • Sperm acrosomal membrane-associated protein 14 antibody
    • Sperm acrosome membrane-associated protein 4 antibody
    see all

Images

  • ab122758, at 1/200 dilution, staining SPACA4 in paraffin-embedded Human testis tissue by Immunohistochemistry.
  • Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
    Lane 2: Negative control (vector only transfected HEK293T lysate)
    Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.

References

ab122758 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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