Overview

  • Product nameAnti-SPAG11B antibody
  • Description
    Rabbit polyclonal to SPAG11B
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    A synthetic peptide corresponding to a region of Human SPAG11B

  • Positive control
    • Fetal muscle lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab75204 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 11 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1:312500.

Target

  • RelevanceSPAG11B belongs to the SPAG11 family. The specific function of SPAG11B has not been determined, but it is thought to be involved in sperm maturation. It is specifically expressed in caput and proximal corpus of epididymis and is present in the epididymal epithelium and on the sperm surface, with a subacrosomal equatorial distribution on the sperm head. There are two named isoforms.
  • Cellular localizationSecreted
  • Database links
  • Alternative names
    • EP2 antibody
    • EP2C antibody
    • EP2D antibody
    • Epididymal protein 2 antibody
    • HE2 antibody
    • HE2C antibody
    • Protein EP2 antibody
    • SPAG11 antibody
    • Sperm antigen HE2 antibody
    • Sperm associated antigen 11 antibody
    • Sperm associated antigen 11B antibody
    see all

Anti-SPAG11B antibody images

  • Anti-SPAG11B antibody (ab75204) at 1 µg/ml + fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 11 kDa
    Observed band size : 16 kDa (why is the actual band size different from the predicted?)

References for Anti-SPAG11B antibody (ab75204)

ab75204 has not yet been referenced specifically in any publications.

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