The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml.
1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionSeems to be important for the structural integrity of the central apparatus of the sperm axoneme.
Tissue specificityHighly expressed in testis. Expressed in organs that contain cilia-bearing cells including brain, oviduct, lung, and uterus.
Cellular localizationCytoplasm. Cytoplasm > cytoskeleton > flagellum axoneme. Detected in the cytoplasm of round spermatids and in condensing spermatids. Localized to the central pair of the sperm flagellar axoneme. Colocalizes with SPAG6 on microtubules.