Overview

  • Product nameAnti-SPAG4 antibody
    See all SPAG4 primary antibodies
  • Description
    Mouse monoclonal to SPAG4
  • Tested applicationsSuitable for: WB, ELISA, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 221-331 of Human SPAG4 with a proprietary tag (NP_003107).

  • Positive control
    • Recombinant Human SPAG4 Protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab118100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 48 kDa. This antibody has only been tested against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
ELISA Use at an assay dependent dilution.
Sandwich ELISA Use a concentration of 10 µg/ml.

Target

  • FunctionMay assist the organization and assembly of outer dense fibers (ODFs), a specific structure of the sperm tail.
  • Sequence similaritiesContains 1 SUN domain.
  • Cellular localizationMembrane. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > flagellum axoneme. In spermatids, it is localized in the transient manchette and in the axoneme of elongating spermatids and epididymal sperm.
  • Information by UniProt
  • Database links
  • Alternative names
    • acrosomal protein ACR55 antibody
    • cancer/testis antigen 127 antibody
    • CT127 antibody
    • OTTHUMP00000030768 antibody
    • OTTHUMP00000204168 antibody
    • Outer dense fiber-associated protein SPAG4 antibody
    • Sad1 and UNC84 domain containing 4 antibody
    • Spag4 antibody
    • SPAG4_HUMAN antibody
    • Sperm associated antigen 4 antibody
    • sperm tail protein antibody
    • Sperm-associated antigen 4 protein antibody
    • SUN domain-containing protein 4 antibody
    • SUN4 antibody
    see all

Anti-SPAG4 antibody images

  • Anti-SPAG4 antibody (ab118100) at 5 µg/ml + Recombinant Protein at 0.2 µg
    Developed using the ECL technique

    Predicted band size : 48 kDa
  • ab118100 at 10 µg/ml as a capture antibody. The detection limit for recombinant tagged SPAG4 is 1 ng/ml.

References for Anti-SPAG4 antibody (ab118100)

ab118100 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. As stated on the datasheet, we have no data on the detection of endogenouse protein for this product. If you did chosee to test the product in endogenous samples, since product is to be used in an untested species or applic...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"