Overview

  • Product nameAnti-SPAG6 antibody
    See all SPAG6 primary antibodies
  • Description
    Mouse monoclonal to SPAG6
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment: MSQRQVLQVF EQYQKARTQF VQMVAELATR PQNIETLQNA GVMSLLRTLL LDVVPTIQQT AALALGRLAN YNDDLAEAVV KCDILPQLVY SLAEQNRFYK , corresponding to amino acids 1-101 of Human SPAG6

Properties

Applications

Our Abpromise guarantee covers the use of ab56778 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionImportant for structural integrity of the central apparatus in the sperm tail and for flagellar motility.
    • Tissue specificityHighly expressed in testis.
    • Sequence similaritiesContains 8 ARM repeats.
    • Cellular localizationCytoplasm > cytoskeleton. Cell projection > cilium > flagellum. Associated with microtubules. Detected on the sperm flagellum.
    • Information by UniProt
    • Database links
    • Alternative names
      • axoneme central apparatus protein antibody
      • DKFZp434I153 antibody
      • MGC26276 antibody
      • PF16 antibody
      • PF16 protein homolog antibody
      • Protein PF16 homolog antibody
      • Repro SA 1 antibody
      • Repro-SA-1 antibody
      • SPAG6 antibody
      • SPAG6_HUMAN antibody
      • Sperm associated antigen 6 antibody
      • Sperm flagellar protein antibody
      • Sperm-associated antigen 6 antibody
      see all

    Anti-SPAG6 antibody images



    • Predicted band size : 55 kDa
      SPAG6 antibody (ab56778) at 1ug/lane + A-431 cell lysate at 25ug/lane.
    • Anti-SPAG6 antibody (ab56778) at 1/500 dilution + whole tissue lysate prepared from mouse lung at 80 µg

      Secondary
      Sheep anti-mouse conjugated to HRP at 1/10000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 55 kDa
      Observed band size : 55 kDa


      Exposure time : 20 minutes

      This image is courtesy of an anonymous abreview.

      See Abreview

    References for Anti-SPAG6 antibody (ab56778)

    ab56778 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (Lung)
    Loading amount 80 µg
    Specification Lung
    Gel Running Conditions Reduced Denaturing (10% PAGE)
    Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
    Username

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    Verified customer

    Submitted Feb 18 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"