Overview

  • Product nameAnti-SPAG8 antibody
    See all SPAG8 primary antibodies
  • Description
    Mouse monoclonal to SPAG8
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length human SPAG8 with tag (aa 1-502; AAH19247).

  • Positive control
    • SPAG8 transfected 293T cell lysate; Recombinant full length of human SPAG8.

Properties

Applications

Our Abpromise guarantee covers the use of ab77430 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 53 kDa).
ELISA Use at an assay dependent dilution.

Target

  • FunctionMay play a role in fertility and microtubule formation through interaction with RANBP9.
  • Tissue specificityExpressed in testis (germ cells), but not in liver, kidney, prostate and small intestine.
  • Cellular localizationCytoplasmic vesicle > secretory vesicle > acrosome. Acrosomal region of the head of mature sperm cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • BS 84 antibody
    • HSD 1 antibody
    • HSD-1 antibody
    • HSD1 antibody
    • hSMP1 antibody
    • MGC26201 antibody
    • MH SPAG8 antibody
    • OTTHUMP00000021352 antibody
    • OTTMUSP00000032209 antibody
    • SMP-1 antibody
    • SMP1 antibody
    • SPAG3 antibody
    • SPAG8 antibody
    • SPAG8_HUMAN antibody
    • Sperm associated antigen 8 antibody
    • Sperm associated antigen 8 like protein antibody
    • Sperm membrane protein 1 antibody
    • Sperm membrane protein BS-84 antibody
    • Sperm-associated antigen 8 antibody
    see all

Anti-SPAG8 antibody images

  • Anti-SPAG8 antibody (ab77430) at 5 µg/ml + Recombinant full length human SPAG8 at 0.2 µg

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

    Predicted band size : 53 kDa
    Observed band size : 81 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-SPAG8 antibody (ab77430) at 5 µg/ml

    Lane 1 : SPAG8 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 53 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)

References for Anti-SPAG8 antibody (ab77430)

ab77430 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"