Overview

  • Product nameAnti-SPARC antibody
    See all SPARC primary antibodies
  • Description
    Rabbit polyclonal to SPARC
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide derived from the human SPARC protein.

  • Positive control
    • Mouse heart lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab55847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa). PubMed: 19509023

Target

  • FunctionAppears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.
  • Sequence similaritiesBelongs to the SPARC family.
    Contains 1 EF-hand domain.
    Contains 1 follistatin-like domain.
    Contains 1 Kazal-like domain.
  • Developmental stageExpressed at high levels in tissues undergoing morphogenesis, remodeling and wound repair.
  • Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane. In or around the basement membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AA517111 antibody
    • Basement membrane protein 40 antibody
    • Basement-membrane protein 40 antibody
    • BM 40 antibody
    • BM-40 antibody
    • BM40 antibody
    • Cysteine rich protein antibody
    • hm:zeh0062 antibody
    • MGC128090 antibody
    • OI17 antibody
    • ON antibody
    • Osteonectin antibody
    • Secreted acidic cystein rich glycoprotein antibody
    • Secreted protein acidic and rich in cysteine antibody
    • Secreted protein acidic cysteine rich (osteonectin) antibody
    • Secreted protein acidic cysteine rich antibody
    • SPARC antibody
    • SPRC antibody
    • SPRC_HUMAN antibody
    see all

References for Anti-SPARC antibody (ab55847)

This product has been referenced in:
  • Liu Y  et al. Apratoxin a reversibly inhibits the secretory pathway by preventing cotranslational translocation. Mol Pharmacol 76:91-104 (2009). Read more (PubMed: 19403701) »
  • Kos K  et al. Regulation of the fibrosis and angiogenesis promoter SPARC/osteonectin in human adipose tissue by weight change, leptin, insulin, and glucose. Diabetes 58:1780-8 (2009). WB ; Human . Read more (PubMed: 19509023) »

See all 2 Publications for this product

Product Wall

Thank you for your enquiry. The 75kDa band could be due to insufficient boiling of the sample, which resulted in the 75kDa dimer band. Since this image is confusing, I have removed it from the datasheet. Please rest assured that the product does de...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"