• Product nameAnti-SPARCL1 antibody
    See all SPARCL1 primary antibodies
  • Description
    Rabbit polyclonal to SPARCL1
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, between amino acids 215-244 from the Central region of Human SPARCL1 (NP_001121782.1, NP_004675.3).

  • Positive control
    • NCI-H460 cell line lysate, Human brain tissue


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab107533 is purified through a protein A column, followed by peptide affinity purification.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab107533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 75 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • Tissue specificityHighly expressed in lymph node, brain, heart, lung, skeletal muscle, ovary, small intestine, and colon, with lower levels in placenta, pancreas, testis, spleen, and thymus, and no expression in kidney, liver, and peripheral blood leukocytes.
  • Sequence similaritiesBelongs to the SPARC family.
    Contains 1 EF-hand domain.
    Contains 1 follistatin-like domain.
    Contains 1 Kazal-like domain.
  • Post-translational
    N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. Thr-398 is the preferred site to Thr-399.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ecm2 antibody
    • Extracellular matrix protein 2 antibody
    • Hevin antibody
    • High endothelial venule protein antibody
    • MAST 9 antibody
    • mast9 antibody
    • Matrix glycoprotein Sc1 antibody
    • PIG33 antibody
    • proliferation inducing protein 33 antibody
    • RAGS1 antigen antibody
    • SC1 antibody
    • SPARC like 1 antibody
    • SPARC-like protein 1 antibody
    • Sparcl1 antibody
    • SPRL1_HUMAN antibody
    see all

Anti-SPARCL1 antibody images

  • Anti-SPARCL1 antibody (ab107533) at 1/100 dilution + NCI-H460 cell line lysate at 35 µg

    Predicted band size : 75 kDa
  • Immunohistochemistry analysis in formalin fixed and paraffin embedded Human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. ab107533 at a dilution of 1/50.

References for Anti-SPARCL1 antibody (ab107533)

This product has been referenced in:
  • Hurley PJ  et al. Secreted protein, acidic and rich in cysteine-like 1 (SPARCL1) is down regulated in aggressive prostate cancers and is prognostic for poor clinical outcome. Proc Natl Acad Sci U S A 109:14977-82 (2012). IHC-P . Read more (PubMed: 22927397) »

See 1 Publication for this product

Product Wall

Thank you for your inquiry.

I am sorry to confirm that we do not expect a cross reactivity of ab107533 with rat.

The immunogen only shows a 52% homology and we only expect a cross reactivity form 85% homology.

I am sorry t...

Read More