Anti-Spasmolytic Polypeptide antibody [GE16C] (ab49536)

Overview

  • Product nameAnti-Spasmolytic Polypeptide antibody [GE16C]
    See all Spasmolytic Polypeptide primary antibodies
  • Description
    Mouse monoclonal [GE16C] to Spasmolytic Polypeptide
  • Tested applicationsSuitable for: IHC-Pmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to a 16 amino acid C-terminal sequence of the human spasmolytic polypeptide.

  • Positive control
    • Human stomach and duodenum tissue

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: Tissue culture supernatant
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberGE16C
  • MyelomaP3-NS1/1-Ag4-1
  • IsotypeIgM
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab49536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notesIs unsuitable for WB.
  • Target

    • FunctionInhibits gastrointestinal motility and gastric acid secretion. Could function as a structural component of gastric mucus, possibly by stabilizing glycoproteins in the mucus gel through interactions with carbohydrate side chains.
    • Tissue specificityStomach.
    • Sequence similaritiesContains 2 P-type (trefoil) domains.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • SML 1 antibody
      • SML1 antibody
      • SP antibody
      • Spasmolysin antibody
      • Spasmolytic polypeptide antibody
      • spasmolytic protein 1 antibody
      • TFF 2 antibody
      • TFF2 antibody
      • TFF2_HUMAN antibody
      • trefoil factor 2 (spasmolytic protein 1) antibody
      • Trefoil factor 2 antibody
      • Trefoil factor 2 precursor antibody
      see all

    Anti-Spasmolytic Polypeptide antibody [GE16C] images

    • Immunohistochemical staining of paraffin embedded human stomach tissue, using ab49536 at 1/25 dilution, following antigen retrieval. 60 minutes primary antibody incubation at 25oC and standard ABC technique used. Note cytoplasmic staining of cardiac glands.

    References for Anti-Spasmolytic Polypeptide antibody [GE16C] (ab49536)

    This product has been referenced in:
    • Wu CS  et al. Aberrant JAK/STAT Signaling Suppresses TFF1 and TFF2 through Epigenetic Silencing of GATA6 in Gastric Cancer. Int J Mol Sci 17:N/A (2016). Read more (PubMed: 27598141) »
    • Nam KT  et al. Gastric tumor development in Smad3-deficient mice initiates from forestomach/glandular transition zone along the lesser curvature. Lab Invest 92:883-95 (2012). Mouse . Read more (PubMed: 22411066) »

    See all 4 Publications for this product

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    Thank you for contacting us. I have placed the protocol below. Please let me know if you have any further questions.



    Cut and mount sections on slides coated with a suitable tissue adhesive.1.

    Deparaffinize sections and rehy...

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    This kit will work with mouse IgM antiobodies as well.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoc...

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    ab127055 kit can be used with any mouse antibody - this mean the kit would be suitable.

    Please note ab49536 is tested and guaranteed to be with mouse species however ab57752 is only tested with human sampl...

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    Thank you for your enquiry.

    According to the developer of the kit, the mouse-on-mouse IHC kit will be effective with IgM primary antibodies. The anti-Spamolytic Polypeptide antibody ab49536 is IgM isotype.

    Please contact us if you...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"