Overview

  • Product nameAnti-Spastin antibody
    See all Spastin primary antibodies
  • Description
    Mouse monoclonal to Spastin
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 200 -305 of Human Spastin (NP_055761) with a proprietary tag

  • Positive control
    • Jurkat cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab89449 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 68 kDa.
ELISA Use at an assay dependent dilution.

Target

  • RelevanceSpastin is thought have a role in microtubule dynamics through its function as a microtubule severing protein. It is localised to the centrosome of neuronal cells but is not found in glial cells. Mutation in the ATPase binding domain of spastin causes hereditary spastic paraplegias (HSP), a large group of clinically similar disorders. Mutant forms of spastin are generally found throughout the cytoplasm rather then within the nucleus.
  • Cellular localizationCytoplasm, Cytoskeleton, Endoplasmic reticulum, Endosome, Membrane, Microtubule, Nucleus
  • Database links
  • Alternative names
    • ADPSP antibody
    • FSP 2 antibody
    • FSP2 antibody
    • KIAA1083 antibody
    • Spast antibody
    • spastic paraplegia 4 (autosomal dominant; spastin) antibody
    • Spastic paraplegia 4 antibody
    • spastin antibody
    • SPG 4 antibody
    • SPG4 antibody
    • SPG4 gene antibody
    see all

Anti-Spastin antibody images

  • Anti-Spastin antibody (ab89449) at 1 µg/ml + Recombinant Spastin Protein (Immunogen) at 0.2 µg

    Predicted band size : 68 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Anti-Spastin antibody (ab89449) at 1 µg/ml + Jurkat cell lysate at 50 µg

    Predicted band size : 68 kDa
    Observed band size : 68 kDa

References for Anti-Spastin antibody (ab89449)

ab89449 has not yet been referenced specifically in any publications.

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