Overview

  • Product nameAnti-SPATA13 antibody
    See all SPATA13 primary antibodies
  • Description
    Rabbit polyclonal to SPATA13
  • Tested applicationsSuitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: ALRPAEWGTL DGSDLEDTDD AFQRSTHRSR SLRRAYGLGR ICLLDAPQNH ATPTIATGQV PAVCEILVRD PENNSMGYRR SKSTDNLAFL KKSSFKRKST SNLADLRTAH of Human SPATA13

  • Positive control
    • Human duodenum tissue; U 251 MG cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab122627 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. using PFA/Triton X-100 Fixation/Permeabilization.

Target

  • FunctionActs as guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Regulates cell migration and adhesion assembly and disassembly through a RAC1, PI3K, RHOA and AKT1-dependent mechanism. Increases both RAC1 and CDC42 activity, but decreases the amount of active RHOA. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Involved in tumor angiogenesis and may play a role in intestinal adenoma formation and tumor progression.
  • Tissue specificityExpressed at high levels in the placenta, spleen and kidney, at moderate levels in lung, small intestine, liver, brain and heart, and at low levels in skeletal muscle. Expression is aberrantly enhanced in most colorectal tumors.
  • Sequence similaritiesContains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
    Contains 1 SH3 domain.
  • DomainThe C-terminal tail is required for its GEF activity.
  • Cellular localizationCytoplasm. Cell projection > filopodium. Cell projection > lamellipodium. Cell projection > ruffle membrane. Accumulates in the lamellipodium and ruffle membrane in response to hepatocyte growth factor (HGF) treatment.
  • Information by UniProt
  • Database links
  • Alternative names
    • APC-stimulated guanine nucleotide exchange factor 2 antibody
    • ARHGEF29 antibody
    • Asef2 antibody
    • FLJ31208 antibody
    • RP11-307N16.3 antibody
    • Spata13 antibody
    • Spermatogenesis-associated protein 13 antibody
    • SPT13_HUMAN antibody
    see all

Anti-SPATA13 antibody images

  • ab122627 at 1/200 dilution staining SPATA13 in paraffin-embedded Human duodenum tissue by Immunohistochemistry.
  • ab122627 at 4 µg/ml staining SPATA13 in PFA/Triton X-100 fixed/permeabilized U 251 MG cells by Immunofluorescence (green).

References for Anti-SPATA13 antibody (ab122627)

ab122627 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"