Overview

  • Product nameAnti-SPATA13 antibody
    See all SPATA13 primary antibodies
  • Description
    Rabbit polyclonal to SPATA13
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    antigen: NQKKLAMLNA QKAGHGKSKG YNRCPVAPPH QGLHPIHQRH ITMPTSVPQQ QVFGLAEPKR KSSLFWHTFN RLT, corresponding to amino acids 576-648 of Human SPATA13 Isoforms 1,2,3 and 5 (Q96N96). This is partially absent in Isoform 4.

  • Positive control
    • RT-4 or U-251 MG lysate. Human bronchus tissue. Human A-431 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab122701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250 - 1/500.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. The following steps are recommended prior to antibody incubation: cells fixation in ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permeabilization with 0.1% Triton X-100 in PBS for 3x5 minutes.

Target

  • FunctionActs as guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Regulates cell migration and adhesion assembly and disassembly through a RAC1, PI3K, RHOA and AKT1-dependent mechanism. Increases both RAC1 and CDC42 activity, but decreases the amount of active RHOA. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Involved in tumor angiogenesis and may play a role in intestinal adenoma formation and tumor progression.
  • Tissue specificityExpressed at high levels in the placenta, spleen and kidney, at moderate levels in lung, small intestine, liver, brain and heart, and at low levels in skeletal muscle. Expression is aberrantly enhanced in most colorectal tumors.
  • Sequence similaritiesContains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
    Contains 1 SH3 domain.
  • DomainThe C-terminal tail is required for its GEF activity.
  • Cellular localizationCytoplasm. Cell projection > filopodium. Cell projection > lamellipodium. Cell projection > ruffle membrane. Accumulates in the lamellipodium and ruffle membrane in response to hepatocyte growth factor (HGF) treatment.
  • Information by UniProt
  • Database links
  • Alternative names
    • APC-stimulated guanine nucleotide exchange factor 2 antibody
    • ARHGEF29 antibody
    • Asef2 antibody
    • FLJ31208 antibody
    • RP11-307N16.3 antibody
    • Spata13 antibody
    • Spermatogenesis-associated protein 13 antibody
    • SPT13_HUMAN antibody
    see all

Anti-SPATA13 antibody images

  • All lanes : Anti-SPATA13 antibody (ab122701) at 1/250 dilution

    Lane 1 : RT-4 lysate
    Lane 2 : U251-MG lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Secondary
    Secondary antibody conjugated to horseradish peroxidase at 1/3000 dilution
  • ab122701, at 1/50 dilution, staining SPATA13 in Paraffin-embedded Human bronchus tissue by Immunohistochemistry
  • Immunofluorescent staining of Human cell line A-431 using ab122701 at 4µg/ml. Antibody staining is shown in green.

References for Anti-SPATA13 antibody (ab122701)

ab122701 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"