Overview

  • Product nameAnti-SPATA20 antibody
    See all SPATA20 primary antibodies
  • Description
    Rabbit polyclonal to SPATA20
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 647-756 of Human SPATA20 (Q8TB22).

  • Positive control
    • Human testis tissue. RT-4 and U-251 MG cell lysate. Human plasma lysate. Human liver and tonsil tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab121918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • FunctionMay play a role in fertility regulation.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • SPATA20 antibody
    • Sperm protein SSP411 antibody
    • Sperm-specific protein 411 antibody
    • Spermatogenesis associated 20 antibody
    • Spermatogenesis-associated protein 20 antibody
    • SPT20_HUMAN antibody
    • Ssp411 antibody
    • Tisp78 antibody
    • Transcript increased in spermiogenesis 78 antibody
    see all

Anti-SPATA20 antibody images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus. Recommended concentration of ab121918 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121918 at 1/50 dilution staining SPATA20 in paraffin-embedded Human testis tissue by Immunohistochemistry.
  • All lanes : Anti-SPATA20 antibody (ab121918) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver tissue lysate
    Lane 5 : Human tonsil tissue lysate

    Developed using the ECL technique

References for Anti-SPATA20 antibody (ab121918)

ab121918 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"