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Rabbit polyclonal to SPATA20
antigen sequence, corresponding to amino acids 647-756 of Human SPATA20 (Q8TB22).
- Human testis tissue. RT-4 and U-251 MG cell lysate. Human plasma lysate. Human liver and tonsil tissue lysate.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/250 - 1/500.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
May play a role in fertility regulation.
Information by UniProt
- SPATA20 antibody
- Sperm protein SSP411 antibody
- Sperm-specific protein 411 antibody
Immunocytochemistry/ Immunofluorescence - Anti-SPATA20 antibody (ab121918)
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus. Recommended concentration of ab121918 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPATA20 antibody (ab121918)
ab121918 at 1/50 dilution staining SPATA20 in paraffin-embedded Human testis tissue by Immunohistochemistry.
Western blot - Anti-SPATA20 antibody (ab121918)
All lanes : Anti-SPATA20 antibody (ab121918) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Developed using the ECL technique
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"