Overview

  • Product nameAnti-SPATA21 antibody
    See all SPATA21 primary antibodies
  • Description
    Rabbit polyclonal to SPATA21
  • Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 340-426 (LKEGTCKAQE MEAAVGRLRL QKLPYNPQQE ESSEVPERKV LSILSRLKQQ NYAPNLQSPY AQVPCILLCP QLDKKMVRRQ PSNHYAL) of Human SPATA21.

  • Positive control
    • Human heart muscle tissue; RT-4, U-251 MG, Human plasma, liver and tonsil lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab122204 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Anti-SPATA21 antibody images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in plasma membrane, cytoplasm and vesicles. Recommended concentration of ab122204 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122204 at 1/150 dilution staining SPATA21 in Paraffin-embedded Human Heart muscle tissue by Immunohistochemistry.

References for Anti-SPATA21 antibody (ab122204)

ab122204 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"