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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/250 - 1/500.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Information by UniProt
- SPAT4_HUMAN antibody
- SPATA4 antibody
- SPEF1B antibody
Anti-SPATA4 antibody images
Immunocytochemistry/ Immunofluorescence - Anti-SPATA4 antibody (ab121594)
Immunofluorescent staining of Human cell line U-251MG shows positivity in nuclei but not nucleoli. Recommended concentration of ab121594 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPATA4 antibody (ab121594)
ab121594, at a 1/50 dilution, staining SPATA4 in paraffin embedded Human testis tissue by Immunohistochemistry.
Western blot - Anti-SPATA4 antibody (ab121594)
All lanes : Anti-SPATA4 antibody (ab121594) at 1/250 dilution
Lane 1 : Molecular weight marker
Lane 2 : RT-4 lysate
Lane 3 : U251-MG lysate
Lane 4 : Human plasma lysate
Lane 5 : Human liver lysate
Lane 6 : Human tonsil lysate
References for Anti-SPATA4 antibody (ab121594)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"