Recombinant
RabMAb

Anti-SPC24 antibody [EPR11547(B)] (ab157184)

Overview

  • Product name
    Anti-SPC24 antibody [EPR11547(B)]
    See all SPC24 primary antibodies
  • Description
    Rabbit monoclonal [EPR11547(B)] to SPC24
  • Tested applications
    Suitable for: WB, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human SPC24 (UniProt: Q8NBT2).

  • Positive control
    • 293T, MOLT4, TF1 and HeLa cell lysates, Human thymus tissue lysate; 293T cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab157184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 22 kDa.
ICC/IF 1/50 - 1/100.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Function
      Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
    • Sequence similarities
      Belongs to the SPC24 family.
    • Cellular localization
      Nucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ90806 antibody
      • hSpc24 antibody
      • Kinetochore protein Spc24 antibody
      • SPBC24 antibody
      • spc24 antibody
      • SPC24 NDC80 kinetochore complex component antibody
      • SPC24 NDC80 kinetochore complex component homolog antibody
      • SPC24, NDC80 kinetochore complex component, homolog (S. cerevisiae) antibody
      • SPC24_HUMAN antibody
      • Spindle pole body component 24 homolog (S. cerevisiae) antibody
      • Spindle pole body component 24 homolog antibody
      see all

    Images

    • All lanes : Anti-SPC24 antibody [EPR11547(B)] (ab157184) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : MOLT4 cell lysate
      Lane 3 : TF1 cell lysate
      Lane 4 : HeLa cell lysate
      Lane 5 : Human thymus tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 22 kDa
    • Immunofluorescent analysis of 293T cells, labeling SPC24 with ab157184 at 1/50 dilution.

    References

    ab157184 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% TritonX100 in PBS
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Username

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Jan 05 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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