Recombinant
RabMAb

Anti-SPC24 antibody [EPR11548(B)] (ab169786)

Overview

  • Product name
    Anti-SPC24 antibody [EPR11548(B)]
    See all SPC24 primary antibodies
  • Description
    Rabbit monoclonal [EPR11548(B)] to SPC24
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, ICC/IFmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human SPC24 (UniProt ID: Q8NBT2).

  • Positive control
    • HeLa, 293T, MOLT4, 293 and TF1 cell lysates.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab169786 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 22 kDa.
IP 1/10 - 1/100.
ICC/IF 1/200.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Function
      Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
    • Sequence similarities
      Belongs to the SPC24 family.
    • Cellular localization
      Nucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ90806 antibody
      • hSpc24 antibody
      • Kinetochore protein Spc24 antibody
      • SPBC24 antibody
      • spc24 antibody
      • SPC24 NDC80 kinetochore complex component antibody
      • SPC24 NDC80 kinetochore complex component homolog antibody
      • SPC24, NDC80 kinetochore complex component, homolog (S. cerevisiae) antibody
      • SPC24_HUMAN antibody
      • Spindle pole body component 24 homolog (S. cerevisiae) antibody
      • Spindle pole body component 24 homolog antibody
      see all

    Images

    • ab169786 staining SPC24 in the HeLa cell line from Human cervical cancer by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol. Samples were incubated with primary antibody (1/200 in PBS) for 16 hours at 22°C. ab150081 an Alexa Fluor®488-conjugated Goat anti-rabbit IgG H&L polyclonal(1/200) was used as the secondary antibody. Nuclei were stained with DAPI

      See Abreview

    • All lanes : Anti-SPC24 antibody [EPR11548(B)] (ab169786) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : MOLT4 cell lysate
      Lane 4 : TF1 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 22 kDa

    • Detection of SPC24 by Western Blot of Immunprecipitate. 293 cell lysate (1) or 1X PBS (2) immunoprecipitated using ab169786 at 1/10 dilution; HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    References

    This product has been referenced in:

    See all 2 Publications for this product

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Permeabilization
    No
    Fixative
    Methanol
    Username

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Nov 05 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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