Overview

  • Product nameAnti-SPC25 antibody
    See all SPC25 primary antibodies
  • Description
    Rabbit polyclonal to SPC25
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal amino acids 79-128 (KDNLLKLIAE VKGKKQELEV LTANIQDLKE EYSRKKETIS TANKANAERL) of Human SPC25 (NP_065726).

  • Positive control
    • 721_B cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab116328 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionActs as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
  • Sequence similaritiesBelongs to the SPC25 family.
  • Cellular localizationNucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore.
  • Information by UniProt
  • Database links
  • Alternative names
    • AD024 antibody
    • hSpc25 antibody
    • Kinetochore protein Spc25 antibody
    • SPBC25 antibody
    • SPC25 antibody
    • SPC25_HUMAN antibody
    • spindle pole body component 25 homolog antibody
    see all

Anti-SPC25 antibody images

  • Anti-SPC25 antibody (ab116328) at 1 µg/ml + 721_B cell lysate at 10 µg

    Predicted band size : 26 kDa

References for Anti-SPC25 antibody (ab116328)

ab116328 has not yet been referenced specifically in any publications.

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