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Rabbit polyclonal to SPDYA
Predicted to work with:
Rat, Cow, Pig
Recombinant fragment, corresponding to a region within amino acids 1-313 of Human SPDYA (UniProt: Q5MJ70).
NT2D1, PC3, U-87 MG and SK-N-SH whole cell lysates; Human BT483 xenograft tissue.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 78% PBS, 20% Glycerol, 1% BSA
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 36 kDa.
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
SPDYA (speedy homolog A (Drosophila)) regulates the G1/S phase transition of the cell cycle by binding and activating CDC2, CDK2 and CDKN1B/KIP1. SPDYA can activate CDK2 without promoting CDK2 phosphorylation. SPDYA mediates cell survival during the DNA damage process through activation of CDK2.
hSpy/Ringo A antibody
Western blot - Anti-SPDYA antibody (ab153965)
All lanes : Anti-SPDYA antibody (ab153965) at 1/1000 dilution Lane 1 : NT2D1 whole cell lysate Lane 2 : PC3 whole cell lysate Lane 3 : U-87 MG whole cell lysate Lane 4 : SK-N-SH whole cell lysate Lysates/proteins at 30 µg per lane. Predicted band size: 36 kDa 10% SDS PAGE
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPDYA antibody (ab153965)
Immunohistochemical analysis of paraffin-embedded Human BT483 xenograft tissue labeling SPDYA with ab153965 at 1/1000 dilution.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"