Overview

  • Product name
  • Description
    Rabbit polyclonal to SPDYA
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Cow, Pig
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-313 of Human SPDYA (UniProt: Q5MJ70).

  • Positive control
    • NT2D1, PC3, U-87 MG and SK-N-SH whole cell lysates; Human BT483 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab153965 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 36 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance
    SPDYA (speedy homolog A (Drosophila)) regulates the G1/S phase transition of the cell cycle by binding and activating CDC2, CDK2 and CDKN1B/KIP1. SPDYA can activate CDK2 without promoting CDK2 phosphorylation. SPDYA mediates cell survival during the DNA damage process through activation of CDK2.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • hSpy/Ringo A antibody
    • MGC110856 antibody
    • MGC57218 antibody
    • OTTHUMP00000082662 antibody
    • OTTHUMP00000082663 antibody
    • OTTHUMP00000082664 antibody
    • Rapid inducer of G2/M progression in oocytes A antibody
    • RINGO A antibody
    • RINGO3 antibody
    • SPDY1 antibody
    • Speedy A antibody
    • Speedy homolog 1 (Drosophila) antibody
    • Speedy homolog A antibody
    • Speedy homolog A (Xenopus laevis) antibody
    • Speedy-1 antibody
    • SPY1 antibody
    see all

Images

  • All lanes : Anti-SPDYA antibody (ab153965) at 1/1000 dilution

    Lane 1 : NT2D1 whole cell lysate
    Lane 2 : PC3 whole cell lysate
    Lane 3 : U-87 MG whole cell lysate
    Lane 4 : SK-N-SH whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 36 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human BT483 xenograft tissue labeling SPDYA with ab153965 at 1/1000 dilution.

References

ab153965 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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