Anti-SPDYE1 antibody - C-terminal (ab170181)

Overview

  • Product name
    Anti-SPDYE1 antibody - C-terminal
  • Description
    Rabbit polyclonal to SPDYE1 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human SPDYE1 aa 249-279 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q8NFV5

  • Positive control
    • HepG2 cell lysate; HepG2 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab170181 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 41 kDa.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Involvement in disease
    Note=SPDYE1 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
  • Sequence similarities
    Belongs to the Speedy/Ringo family.
  • Information by UniProt
  • Database links
  • Alternative names
    • Putative WBSCR19-like protein 6 antibody
    • Ringo1 antibody
    • SPDE1_HUMAN antibody
    • SPDYE antibody
    • SPDYE1 antibody
    • Speedy E antibody
    • Speedy homolog E1 (Xenopus laevis) antibody
    • Speedy protein E1 antibody
    • Speedy/RINGO cell cycle regulator family member E1 antibody
    • WBSCR19 antibody
    • Williams-Beuren syndrome chromosomal region 19 protein antibody
    see all

Images

  • Flow cytometric analysis of HepG2 cells labeling SPDYE1  with ab170181 at 1/10 dilution (right histogram), negative control cells (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies was used for the analysis.

  • Anti-SPDYE1 antibody - C-terminal (ab170181) at 1/100 dilution + HepG2 cell lysate at 35 µg

    Predicted band size: 41 kDa

References

ab170181 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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