The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Tissue specificityExpressed at very low levels in many tissues, with strongest expression in cerebellum, spinal cord, stomach, pituitary gland, liver, pancreas, salivary gland, kidney, bladder, and heart.
Sequence similaritiesBelongs to the spectrin family. Contains 2 CH (calponin-homology) domains. Contains 1 PH domain. Contains 31 spectrin repeats.
Cellular localizationCytoplasm > cytoskeleton. Detected prominently in the outer segments of photoreceptor rods and cones and in the basolateral membrane and cytosol of gastric epithelial cells.
ab110867 at 1/50 staining Spectrin beta 5 in paraffin-embedded Human lung carcinoma tissue by Immunohistochemistry; left panel shows staining without immunizing peptide right panel shows staining with immunizing peptide.
References for Anti-Spectrin beta 5 antibody (ab110867)
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