Anti-SPEF1 antibody (ab151100)
Key features and details
- Rabbit polyclonal to SPEF1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SPEF1 antibody -
Description
Rabbit polyclonal to SPEF1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to amino acids 31-151 of Human SPEF1 (UniProt Q9Y4P9-2).
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General notes
The antibody solution should be gently mixed before use.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151100 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/250 - 1/500. Predicted molecular weight: 27 kDa.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/250 - 1/500. Predicted molecular weight: 27 kDa. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Cellular localization
Cytoplasm. Cell projection; cilium; flagellum. Cytoplasm; cytoskeleton; cilium axoneme. Note: Present in the tails of developing and epididymal sperm, internal to the fibrous sheath and around the outer dense fibers of the sperm flagellum. Also found at the apical tip of cilia. -
Database links
- Entrez Gene: 25876 Human
- Omim: 610674 Human
- SwissProt: Q9Y4P9 Human
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Alternative names
- C20orf28 antibody
- Calponin homology and microtubule associated protein antibody
- CLAMP antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling SPEF1 with ab151100 at 1/50 dilution.
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All lanes : Anti-SPEF1 antibody (ab151100) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human Plasma lysate
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
Predicted band size: 27 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab151100 has not yet been referenced specifically in any publications.