Anti-SPEN antibody (ab72266)
Key features and details
- Rabbit polyclonal to SPEN
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SPEN antibody
See all SPEN primary antibodies -
Description
Rabbit polyclonal to SPEN -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rabbit, Horse, Guinea pig, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant -
Immunogen
Synthetic peptide corresponding to Human SPEN.
Database link: Q96T58 -
Positive control
- Whole cell lysate from Hela cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72266 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at 2-5 µg/mg of lysate.
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WB |
Use at an assay dependent concentration.
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Notes |
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IP
Use at 2-5 µg/mg of lysate. |
WB
Use at an assay dependent concentration. |
Target
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Relevance
SPEN is an essential corepressor protein, which probably regulates different key pathways such as the Notch pathway. It is a negative regulator of the Notch pathway via its interaction with RBPSUH, which prevents the association between NOTCH1 and RBPSUH, and therefore suppresses the transactivation activity of Notch signaling. SPEN blocks the differentiation of precursor B-cells into marginal zone B-cells. It probably represses transcription via the recruitment of large complexes containing histone deacetylase proteins. SPEN may bind both to DNA and RNA. -
Cellular localization
Nuclear. Associates with chromatin. -
Database links
- Entrez Gene: 23013 Human
- Entrez Gene: 56381 Mouse
- SwissProt: Q96T58 Human
- SwissProt: A2ADB0 Mouse
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Alternative names
- MINT antibody
- Nuclear receptor transcription cofactor antibody
- RBM15C antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab72266 has been referenced in 3 publications.
- Robert-Finestra T et al. SPEN is required for Xist upregulation during initiation of X chromosome inactivation. Nat Commun 12:7000 (2021). PubMed: 34853312
- Li Y et al. SPEN induces miR-4652-3p to target HIPK2 in nasopharyngeal carcinoma. Cell Death Dis 11:509 (2020). PubMed: 32641685
- Korfanty J et al. SPEN protein expression and interactions with chromatin in mouse testicular cells. Reproduction 156:195-206 (2018). PubMed: 29880719