Overview

  • Product nameAnti-SPEN antibody
  • Description
    Rabbit polyclonal to SPEN
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Horse, Guinea pig, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide from a region between residue 1350 and 1400 of human SPEN, NP_055816.2.

  • Positive control
    • Whole cell lysate from Hela cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab72266 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notesIP: Use at 2-5µg/mg of lysate.
    WB: Use at a concentration of 1 µg/ml in enriched samples (ie. immunoprecipitates). Predicted molecular weight: 402 kDa. Not suitable for WB in crude samples.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceSPEN is an essential corepressor protein, which probably regulates different key pathways such as the Notch pathway. It is a negative regulator of the Notch pathway via its interaction with RBPSUH, which prevents the association between NOTCH1 and RBPSUH, and therefore suppresses the transactivation activity of Notch signaling. SPEN blocks the differentiation of precursor B-cells into marginal zone B-cells. It probably represses transcription via the recruitment of large complexes containing histone deacetylase proteins. SPEN may bind both to DNA and RNA.
    • Cellular localizationNuclear. Associates with chromatin.
    • Database links
    • Alternative names
      • MINT antibody
      • Nuclear receptor transcription cofactor antibody
      • RBM15C antibody
      • SHARP antibody
      • SPEN homolog antibody
      • Spen homolog transcriptional regulator antibody
      see all

    Anti-SPEN antibody images

    • Immunoprecipitation/ Western Blot of SPEN.
      Lane 1: ab72266 at 3µg/mg whole cell lysate.
      Lane 2: Control IgG.
      ab72266 at 1µg/ml for WB.
      Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
      Chemiluminescence with an exposure time of 3 minutes.

    References for Anti-SPEN antibody (ab72266)

    ab72266 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you very much for your interest in ab72266. The immunogen for this antibody is 86% homologous with the mouse SPEN protien (UniProt: Q62504). Based on this information, there is a good chance that ab72266 will detect mouse SPEN, but this has not b...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"