Anti-Spermidine antibody (ab7318)
Key features and details
- Rabbit polyclonal to Spermidine
- Suitable for: IHC-P
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-Spermidine antibody -
Description
Rabbit polyclonal to Spermidine -
Host species
Rabbit -
Specificity
Reacts with spermidine and cross reacts with spermine. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Spermidine conjugated to KLH.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7318 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration.
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Notes |
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IHC-P
Use at an assay dependent concentration. |
Target
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Relevance
Spermidine is a polyamine compound was originally isolated from semen. It is found ribosomes and living tissues and has various metabolic functions.
Images
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ab7318 staining Spermidine in rat lungs by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). Tissue underwent fixation in 4% paraformaldehyde overnight and embedded in paraffin, heat mediate antigen retrieval was performed in target retrieval solution and sections were immersed in 3% hydrogen peroxide for 5 min to deplete the endogenous peroxidase activity. Sections were incubated in 1% bovine serum albumin in PBS for 30 min followed by primary antibody at room temperature for 2 h. A primary antibody Enhancer and HRP polymer were used according to the manufacturer's instructions. Left image show staining with ab7318 while the right one show staining with same procedure without primary antibody.
Protocols
Datasheets and documents
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Datasheet download
References (4)
ab7318 has been referenced in 4 publications.
- Bian X et al. AMD1 upregulates hepatocellular carcinoma cells stemness by FTO mediated mRNA demethylation. Clin Transl Med 11:e352 (2021). PubMed: 33783988
- Yang K et al. Measuring non-polyaminated lipocalin-2 for cardiometabolic risk assessment. ESC Heart Fail 4:563-575 (2017). PubMed: 29154418
- Rossi G et al. Immunohistochemical expression of ornithine decarboxylase, diamine oxidase, putrescine, and spermine in normal canine enterocolic mucosa, in chronic colitis, and in colorectal cancer. Biomed Res Int 2015:172756 (2015). IHC-P . PubMed: 26550563
- Liao CP et al. Pneumocystis mediates overexpression of antizyme inhibitor resulting in increased polyamine levels and apoptosis in alveolar macrophages. J Biol Chem 284:8174-84 (2009). IHC-P ; Rat . PubMed: 19158080