Overview

  • Product nameSpermidine hydrochloride
  • Description
    Endogenous polyamine. nNOS inhibitor.
  • Biological descriptionEndogenous polyamine, that binds to the polyamine modulatory site of the NMDA receptor. Inhibits neuronal nitric oxide synthase (nNOS). Binds and precipitates DNA; may be used for purification of DNA binding proteins.
  • Purity> 99%

Properties

    Applications

    Our Abpromise guarantee covers the use of ab120057 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    Spermidine hydrochloride images

    • ab9969 staining IL10 in RAW 246.7 cells treated with spermidine hydrochloride (ab120057), by ICC/IF. Increase in IL10 expression correlates with increased concentration of spermidine hydrochloride, as described in literature.
      The cells were incubated at 37°C for 24 hour in media containing different concentrations of ab120057 (spermidine hydrochloride) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab9969 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

    References for Spermidine hydrochloride (ab120057)

    This product has been referenced in:

    See 1 Publication for this product

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    - The datasheets can be downloaded from the links;

    http://www.abcam.com/Spermine-ab120051.html

    http://www.abcam.com/Spermidine-hydrochloride-ab120057.html

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