Anti-Spermidine synthase antibody (ab111884)

Overview

  • Product nameAnti-Spermidine synthase antibody
    See all Spermidine synthase primary antibodies
  • Description
    Rabbit polyclonal to Spermidine synthase
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 22-209 of Human Spermidine synthase (AAH00309).

  • Positive control
    • 293T and Raji whole cell lysates; Human breast carcinoma tissue. This antibody gave a positive result when used in the following methanol fixed cell lines: MDA-MB-231

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab111884 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 34 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer ( PH8.0).
ICC/IF Use a concentration of 1 µg/ml.

Target

  • FunctionCatalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM). Has a strong preference for putrescine as substrate, and has very low activity towards 1,3-diaminopropane. Has extremely low activity towards spermidine.
  • PathwayAmine and polyamine biosynthesis; spermidine biosynthesis; spermidine from putrescine: step 1/1.
  • Sequence similaritiesBelongs to the spermidine/spermine synthase family.
    Contains 1 PABS (polyamine biosynthesis) domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • OTTHUMP00000002170 antibody
    • PAPT antibody
    • Putrescine aminopropyltransferase antibody
    • SPDSY antibody
    • SPEE_HUMAN antibody
    • Spermidine synthase 1 antibody
    • Spermidine synthase antibody
    • SPS1 antibody
    • Srm antibody
    • SRML1 antibody
    see all

Anti-Spermidine synthase antibody images

  • ICC/IF image of ab111884 stained MDA-MB-231 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab111884 at 1µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Dylight® 488 goat anti- rabbit (ab96899) IgG (H+L) preadsorbed, used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-Spermidine synthase antibody (ab111884) at 1/5000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size : 34 kDa
    12% SDS PAGE
  • ab111884 at 1/500 dilution staining Spermidine synthase in Formalin-fixed, Paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.

References for Anti-Spermidine synthase antibody (ab111884)

ab111884 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"