Anti-Spermine antibody (ab26975)
Key features and details
- Rabbit polyclonal to Spermine
- Suitable for: ICC/IF, WB
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-Spermine antibody -
Description
Rabbit polyclonal to Spermine -
Host species
Rabbit -
Specificity
This antibody reacts with spermine. Cross-reacts with spermidine 100%. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Spermine conjugated to KLH.
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General notes
Polyamines are substrates for transglutaminase enzymes and can be covalently conjugated to proteins. In this case Western Blot and other methods can be able to detect polyaminated proteins.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Primary antibody notes
Polyamines are substrates for transglutaminase enzymes and can be covalently conjugated to proteins. In this case Western Blot and other methods can be able to detect polyaminated proteins. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab26975 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (1) |
Use at an assay dependent concentration.
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WB |
1/1200 - 1/5000.
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Notes |
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ICC/IF
Use at an assay dependent concentration. |
WB
1/1200 - 1/5000. |
Target
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Relevance
Spermine, a polyamine, is a positively charged compound found in living cells and can cause conformational changes in DNA via charge and solubility mechanisms which can affect gene expression cell differentiation. Spermine may be linked to the development of cancer. -
Alternative names
- Spermine antibody
Images
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ab26975 staining spermine in the Human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% formaldehyde for 15 minuts at room temperature (Upper images) or iced methanol of 3 minutes (Lower images) and blocked with 2% BSA for 30 minutes at 27°C. Samples were incubated with primary antibody (1/100) for 1 hour at 27°C. An Alexa Fluor®568-conjugated Donkey anti-rabbit polyclonal (1/1000) was used as the secondary antibody. Samples handled without ab26975 were used as negative controls (Left-hand panels).
Protocols
Datasheets and documents
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Datasheet download
References (5)
ab26975 has been referenced in 5 publications.
- Vila A et al. Synaptic Scaffolds, Ion Channels and Polyamines in Mouse Photoreceptor Synapses: Anatomy of a Signaling Complex. Front Cell Neurosci 15:667046 (2021). PubMed: 34393723
- Do THT et al. Arabidopsis ABCG28 is required for the apical accumulation of reactive oxygen species in growing pollen tubes. Proc Natl Acad Sci U S A 116:12540-12549 (2019). PubMed: 31152136
- Maki K et al. Transglutaminase-catalyzed incorporation of polyamines masks the DNA-binding region of the transcription factor Relish. J Biol Chem 292:6369-6380 (2017). WB . PubMed: 28258224
- Nakajima K et al. KCNJ15/Kir4.2 couples with polyamines to sense weak extracellular electric fields in galvanotaxis. Nat Commun 6:8532 (2015). PubMed: 26449415
- Gaur V et al. Crystal structure and functional insights of hemopexin fold protein from grass pea. Plant Physiol 152:1842-50 (2010). PubMed: 20147493