The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 38 kDa). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Pearce et al, J Biol Chem. 2007 Jul 13;282(28):20104-15.; PMID: 17502376).
FunctionComponent of the ERLIN1/ERLIN2 complex which mediates the endoplasmic reticulum-associated degradation (ERAD) of inositol 1,4,5-trisphosphate receptors (IP3Rs). Also involved in ITPR1 degradation by the ERAD pathway.
Sequence similaritiesBelongs to the band 7/mec-2 family.
Cellular localizationEndoplasmic reticulum membrane. Associated with lipid raft-like domains of the endoplasmic reticulum membrane.
Sánchez-Quiles V et al. Prohibitin deficiency blocks proliferation and induces apoptosis in human hepatoma cells: molecular mechanisms and functional implications. Proteomics10:1609-20 (2010).
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