Overview

  • Product nameAnti-SPG3A antibody
    See all SPG3A primary antibodies
  • Description
    Mouse monoclonal to SPG3A
  • Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: MAKNRRDRNS WGGFSEKTYE WSSEEEEPVK KAGPVQVLIV KDDHSFELDE TALNRILLSE AVRDKEVVAV SVAGAFRKGK SFLMDFMLRY MYNQESVDWV , corresponding to amino acids 1-101 of Human SPG3A

  • Positive control
    • ICC/IF: SKNSH cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab58273 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use 1-2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionGTPase tethering membranes through formation of trans-homooligomer and mediating homotypic fusion of endoplasmic reticulum membranes. Functions in endoplasmic reticulum tubular network biogenesis. May also regulate Golgi biogenesis. May regulate axonal development.
  • Tissue specificityExpressed predominantly in the adult and fetal central nervous system. Measurable expression in all tissues examined, although expression in adult brain is at least 50-fold higher than in other tissues. Detected predominantly in pyramidal neurons in the cerebral cortex and the hippocampus of the brain. Expressed in upper and lower motor neurons (at protein level).
  • Involvement in diseaseDefects in ATL1 are the cause of spastic paraplegia autosomal dominant type 3 (SPG3) [MIM:182600]; also known as Strumpell-Lorrain syndrome. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
  • Sequence similaritiesBelongs to the GBP family. Atlastin subfamily.
  • Cellular localizationEndoplasmic reticulum membrane. Golgi apparatus membrane. Cell projection > axon.
  • Information by UniProt
  • Database links
  • Alternative names
    • AD FSP antibody
    • atl1 antibody
    • ATLA1_HUMAN antibody
    • Atlastin GTPase 1 antibody
    • Atlastin-1 antibody
    • Atlastin1 antibody
    • Brain specific GTP binding protein antibody
    • Brain-specific GTP-binding protein antibody
    • FSP1 antibody
    • GBP-3 antibody
    • GBP3 antibody
    • GTP-binding protein 3 antibody
    • Guanine nucleotide-binding protein 3 antibody
    • Guanylate binding protein 3 antibody
    • hGBP3 antibody
    • HSN1D antibody
    • Spastic paraplegia 3 protein A antibody
    • SPG 3A antibody
    • SPG3 antibody
    • SPG3A antibody
    • SPG3A gene antibody
    see all

Anti-SPG3A antibody images

  • ICC/IF image of ab58273 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58273, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 Goat anti-Mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • SPG3A antibody (ab58273) at 1ug/lane + IMR-32 cell lysate at 25ug/lane.
  • Overlay histogram showing SH-SY5Y cells stained with ab58273 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58273, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-SPG3A antibody (ab58273)

This product has been referenced in:
  • Abrahamsen G  et al. A patient-derived stem cell model of hereditary spastic paraplegia with SPAST mutations. Dis Model Mech 6:489-502 (2013). WB ; Human . Read more (PubMed: 23264559) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"