Anti-Sphingomyelin Synthase 1 antibody (ab235057)
Key features and details
- Rabbit polyclonal to Sphingomyelin Synthase 1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Sphingomyelin Synthase 1 antibody
See all Sphingomyelin Synthase 1 primary antibodies -
Description
Rabbit polyclonal to Sphingomyelin Synthase 1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Pig -
Immunogen
Recombinant fragment corresponding to Human Sphingomyelin Synthase 1 aa 1-150.
Database link: Q86VZ5 -
Positive control
- WB: HeLa and HepG2 whole cell lysate (ab7900). IHC-P: Human pancreatic tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
>95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab235057 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/5000. Predicted molecular weight: 49 kDa.
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IHC-P |
1/200 - 1/500.
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Notes |
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WB
1/500 - 1/5000. Predicted molecular weight: 49 kDa. |
IHC-P
1/200 - 1/500. |
Target
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Relevance
Sphingomyelin synthase 1 is a bidirectional lipid cholinephosphotransferase capable of converting phosphatidylcholine and ceramide to sphingomyelin (SM) and diacylglycerol (DAG) and vice versa. Sphingomyelin synthase 1 is required for cell growth and is also capable of suppressing BAX-mediated apoptosis and preventing cell death in response to stimuli such as hydrogen peroxide, osmotic stress, elevated temperature and exogenously supplied sphingolipids. It may protect against cell death by reversing the stress-inducible increase in levels of proapoptotic ceramide. -
Cellular localization
Golgi Apparatus. Multi-pass membrane protein -
Database links
- Entrez Gene: 259230 Human
- Entrez Gene: 208449 Mouse
- Entrez Gene: 353229 Rat
- Omim: 611573 Human
- SwissProt: Q86VZ5 Human
- SwissProt: Q8VCQ6 Mouse
- SwissProt: Q7TSX5 Rat
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Alternative names
- MOB antibody
- Phosphatidylcholine:ceramide cholinephosphotransferase 1 antibody
- Protein Mob antibody
see all
Images
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All lanes : Anti-Sphingomyelin Synthase 1 antibody (ab235057) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 49 kDa -
Paraffin-embedded human pancreatic tissue stained for Sphingomyelin Synthase 1 using ab235057 at 1/400 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temperature. Primary antibody (1% BSA) was incubated at 4°C overnight. The primary antibody was detected by a biotinylated secondary antibody and visualized using a HRP conjugated SP system.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab235057 has not yet been referenced specifically in any publications.