Overview

  • Product nameAnti-Sphingomyelin Synthase 1 antibody
    See all Sphingomyelin Synthase 1 primary antibodies
  • Description
    Rabbit polyclonal to Sphingomyelin Synthase 1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    A synthetic peptide corresponding to a region within internal amino acids 144 - 193 (SCFVLTTVMI SVVHERVPPK EVQPPLPDTF FDHFNRVQWA FSICEINGMI) of Human Sphingomyelin Synthase 1 (NP_671512)

  • Positive control
    • HeLa cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab83856 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 49 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevanceSphingomyelin synthase 1 is a bidirectional lipid cholinephosphotransferase capable of converting phosphatidylcholine and ceramide to sphingomyelin (SM) and diacylglycerol (DAG) and vice versa. Sphingomyelin synthase 1 is required for cell growth and is also capable of suppressing BAX-mediated apoptosis and preventing cell death in response to stimuli such as hydrogen peroxide, osmotic stress, elevated temperature and exogenously supplied sphingolipids. It may protect against cell death by reversing the stress-inducible increase in levels of proapoptotic ceramide.
  • Cellular localizationGolgi Apparatus. Multi-pass membrane protein
  • Database links
  • Alternative names
    • MOB antibody
    • Phosphatidylcholine:ceramide cholinephosphotransferase 1 antibody
    • Protein Mob antibody
    • SGMS1 antibody
    • SMS 1 antibody
    • SMS1 antibody
    • TMEM23 antibody
    • Transmembrane protein 23 antibody
    see all

Anti-Sphingomyelin Synthase 1 antibody images

  • Anti-Sphingomyelin Synthase 1 antibody (ab83856) at 1 µg/ml + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 49 kDa
    Observed band size : 49 kDa

References for Anti-Sphingomyelin Synthase 1 antibody (ab83856)

This product has been referenced in:
  • Yachi R  et al. Subcellular localization of sphingomyelin revealed by two toxin-based probes in mammalian cells. Genes Cells 17:720-7 (2012). WB ; African Green Monkey . Read more (PubMed: 22747662) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"