Anti-Sphingomyelin Synthase 2 antibody (ab103060)

Overview

  • Product nameAnti-Sphingomyelin Synthase 2 antibody
    See all Sphingomyelin Synthase 2 primary antibodies
  • Description
    Rabbit polyclonal to Sphingomyelin Synthase 2
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, between C-terminal amino acids 336-365 of Human Sphingomyelin Synthase 2.

  • Positive control
    • CEM cell line, Human Brain Tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab103060 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 42 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionBidirectional lipid cholinephosphotransferase capable of converting phosphatidylcholine (PC) and ceramide to sphingomyelin (SM) and diacylglycerol (DAG) and vice versa. Direction is dependent on the relative concentrations of DAG and ceramide as phosphocholine acceptors. Directly and specifically recognizes the choline head group on the substrate. Also requires two fatty chains on the choline-P donor molecule in order to be recognized efficiently as a substrate. Does not function strictly as a SM synthase. Required for cell growth.
  • Tissue specificityBrain, heart, kidney, liver, muscle and stomach.
  • Sequence similaritiesBelongs to the sphingomyelin synthase family.
  • Post-translational
    modifications
    Palmitoylated on Cys-331, Cys-332, Cys-343 and Cys-348; which plays an important role in plasma membrane localization.
  • Cellular localizationCell membrane. Golgi apparatus membrane. Predominantly plasma membrane, some in Golgi apparatus.
  • Information by UniProt
  • Database links
  • Alternative names
    • MGC26963 antibody
    • Phosphatidylcholine:ceramide cholinephosphotransferase 2 antibody
    • SGMS 2 antibody
    • Sgms2 antibody
    • SM synthase antibody
    • SMS 2 antibody
    • SMS2 antibody
    • SMS2_HUMAN antibody
    • Sphingomyelin synthase 2 antibody
    see all

Anti-Sphingomyelin Synthase 2 antibody images

  • Anti-Sphingomyelin Synthase 2 antibody (ab103060) at 1/100 dilution + CEM cell lysate at 35 µg

    Predicted band size : 42 kDa
  • IHC analysis in formalin fixed and paraffin embedded Human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining, using ab103060 at a dilution of 1/50.

References for Anti-Sphingomyelin Synthase 2 antibody (ab103060)

ab103060 has not yet been referenced specifically in any publications.

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