The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWB: Use at a concentration of 1-5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
FunctionBidirectional lipid cholinephosphotransferase capable of converting phosphatidylcholine (PC) and ceramide to sphingomyelin (SM) and diacylglycerol (DAG) and vice versa. Direction is dependent on the relative concentrations of DAG and ceramide as phosphocholine acceptors. Directly and specifically recognizes the choline head group on the substrate. Also requires two fatty chains on the choline-P donor molecule in order to be recognized efficiently as a substrate. Does not function strictly as a SM synthase. Required for cell growth.
Tissue specificityBrain, heart, kidney, liver, muscle and stomach.
Sequence similaritiesBelongs to the sphingomyelin synthase family.
Post-translational modificationsPalmitoylated on Cys-331, Cys-332, Cys-343 and Cys-348; which plays an important role in plasma membrane localization.
Cellular localizationCell membrane. Golgi apparatus membrane. Predominantly plasma membrane, some in Golgi apparatus.
Western blot - Sphingomyelin Synthase 2 antibody (ab58074)
Western blot against tagged recombinant protein immunogen using ab58074 Sphingomyelin Synthase 2 antibody at 1ug/ml. Predicted band size of immunogen is 34 kDa
References for Anti-Sphingomyelin Synthase 2 antibody (ab58074)
This product has been referenced in:
Barceló-Coblijn G et al. Sphingomyelin and sphingomyelin synthase (SMS) in the malignant transformation of glioma cells and in 2-hydroxyoleic acid therapy. Proc Natl Acad Sci U S A108:19569-74 (2011).
Read more (PubMed: 22106271) »