Our Abpromise guarantee covers the use of ab71700 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|IHC-P||1/50 - 1/100.|
|WB||1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 43 kDa).Can be blocked with SPHK1 peptide (ab187382).
There are two known isoforms of this protein, one predicted around 43 kDa and the other around 51 kDa.
Immunohistochemical staining of SPHK1 in Human hepatocarcinoma tissue sections (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections) with ab71700 at a dilution of 1/25. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hours at 38°C. Antigen retrieval was heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A HRP-conjugated goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.
Incubation time was overnight at 4°C. Blocking/Dilution buffer: 5% NFDM/TBST.