Recombinant
RabMAb

Anti-SPHK1 antibody [EPR4293(2)] (ab109522)

Overview

  • Product name
    Anti-SPHK1 antibody [EPR4293(2)]
    See all SPHK1 primary antibodies
  • Description
    Rabbit monoclonal [EPR4293(2)] to SPHK1
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human SPHK1

  • Positive control
    • Raji, JAR, HUVEC or HT-1080 lysate Permeabilized Raji cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab109522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 51 kDa (predicted molecular weight: 43 kDa).
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    Images

    • All lanes : Anti-SPHK1 antibody [EPR4293(2)] (ab109522) at 1/1000 dilution

      Lane 1 : Raji cell lysate
      Lane 2 : JAR cell lysate
      Lane 3 : HUVEC cell lysate
      Lane 4 : HT-1080 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 43 kDa
      Observed band size : 51 kDa (why is the actual band size different from the predicted?)
    • Flow cytometric analysis of permeabilized Raji cells using ab109522 (red), or a rabbit IgG (negative) (green).

    References

    ab109522 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab109522.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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