SPHK1 overexpression 293T lysate (whole cell) (ab94163)

Overview

  • Product nameSPHK1 overexpression 293T lysate (whole cell)
  • Description
    SPHK1 overexpression lysate
  • General notesab94163 is a 293T cell transfected lysate in which Human SPHK1 has been transiently over-expressed using a pCMV-SPHK1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • BackgroundFunction: Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra-and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. Tissue specificity: Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle. Similarity: Contains 1 DAGKc domain.

Associated products

Applications

Our Abpromise guarantee covers the use of ab94163 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

SPHK1 overexpression 293T lysate (whole cell) images

  • ab94163 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-SPHK1 antibody (ab56414) at 1/500 dilution

    Lane 1 : SPHK1 overexpression 293T lysate (whole cell) (ab94163)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for SPHK1 overexpression 293T lysate (whole cell) (ab94163)

ab94163 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"