Anti-SPHK2 antibody (ab92607)

Overview

  • Product nameAnti-SPHK2 antibody
    See all SPHK2 primary antibodies
  • Description
    Rabbit polyclonal to SPHK2
  • SpecificityDetects endogenous levels of total SPHK2.
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthesized peptide corresponding to an internal region of Human SPHK2.

  • Positive control
    • WB: 293 cell lysate; IHC-P: Human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab92607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
IHC-P
  • Application notesELISA: 1/5000.
    IHC-P: 1/50 - 1/100.
    WB: 1/500 - 1/1000. Predicted molecular weight: 69 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Anti-SPHK2 antibody images

    • All lanes : Anti-SPHK2 antibody (ab92607) at 1/500 dilution

      Lane 1 : extracts from 293 cells
      Lane 2 : extracts from 293 cells with immunizing peptide at 10 µg

      Lysates/proteins at 20 µg per lane.


      Predicted band size : 69 kDa
    • Left panel: ab92607, at a 1/50 dilution, staining SPHK2 in formalin-fixed, paraffin-embedded Human breast carcinoma tissue.
      Right panel: same, after pre-incubation with the immunizing phosphopeptide.

    References for Anti-SPHK2 antibody (ab92607)

    ab92607 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Sample Rat Tissue lysate - whole (Whole retina)
    Gel Running Conditions Reduced Denaturing (12%)
    Loading amount 50 µg
    Specification Whole retina
    Blocking step SEA Block Blocking Buffer (Thermo Scientific) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 14 2015

    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (CORTEX)
    Loading amount 50 µg
    Specification CORTEX
    Gel Running Conditions Reduced Denaturing (10%)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 18 2012

    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Rat Cell lysate - whole cell (15 DIV Hippocampal neurons)
    Loading amount 70 µg
    Specification 15 DIV Hippocampal neurons
    Gel Running Conditions Reduced Denaturing (10%)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 18 2012

    Thank you for contacting us. I am sorry thatab92607 is not working in our customer's western blot. We are happy to offer a refund or replacement if the customer has purchased the product in the past six months and is using it in a tested species and ap...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"