Anti-SPHK2 (phospho T614) antibody (ab111948)
Key features and details
- Rabbit polyclonal to SPHK2 (phospho T614)
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-SPHK2 (phospho T614) antibody
See all SPHK2 primary antibodies -
Description
Rabbit polyclonal to SPHK2 (phospho T614) -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- Human Breast carcinoma tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49.1% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS (without Mg2+ and Ca2+) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
ab111948 was affinity-purified from Rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab111948 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
Notes |
---|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra-and extracellular functions. Also acts on D-erythro-dihydrosphingosine, D-erythro-sphingosine and L-threo-dihydrosphingosine. Binds phosphoinositides. -
Sequence similarities
Contains 1 DAGKc domain. -
Cellular localization
Cytoplasm. Membrane and Lysosome membrane. - Information by UniProt
-
Database links
- Entrez Gene: 56848 Human
- Entrez Gene: 56632 Mouse
- Entrez Gene: 308589 Rat
- Omim: 607092 Human
- SwissProt: Q9NRA0 Human
- SwissProt: Q9JIA7 Mouse
- Unigene: 528006 Human
- Unigene: 24222 Mouse
-
Alternative names
- SK 2 antibody
- Sphingosine kinase 2 antibody
- Sphingosine kinase type 2 antibody
see all
Images
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab111948 has not yet been referenced specifically in any publications.