Overview

  • Product nameAnti-Spi2A antibody [MoFo 29.2]
    See all Spi2A primary antibodies
  • Description
    Hamster monoclonal [MoFo 29.2] to Spi2A
  • Specificityab36772 recognises Serine protease inhibitor 2A, or Serpin 2A (spi2A).
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Hamster, Human
  • Immunogen

    Synthetic peptide corresponding to Hamster Spi2A aa 418-436.
    Sequence:

    PERSMNFPNGEGASSQRLC

Properties

Applications

Our Abpromise guarantee covers the use of ab36772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. PubMed: 18240954

Target

  • FunctionSerine and cysteine protease inhibitor. Can inhibit lysosomal papain-like proteases including the cathepsins B, G, H, K, L and V. Ineffective against elastase, granzyme A, granzyme B, or caspases 3, 8 or 9. Inhibition of cytoplasmic cathepsin B following release from the lysosome may protect cells from apoptosis. This may facilitate the survival of progenitor T-cells and the subsequent development of long term memory CD8 T-cells.
  • Tissue specificityExpressed in bone marrow (particularly hematopoietic stem cells), heart, kidney, liver, lung, skeletal muscle, spleen, testis, thymus and T-cells.
  • Sequence similaritiesBelongs to the serpin family.
  • Developmental stageT-cell specific expression rises during the differentiation of CD8 T-cell progenitors into memory CD8 T-cells.
  • DomainThe reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the serpin reactive site and the protease. The resulting inactive serpin-protease complex is highly stable (By similarity). Variability within the reactive center loop (RCL) sequences of Serpina3 paralogs may determine target protease specificity.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Serine protease inhibitor 2A antibody
    • Serine protease inhibitor A3G antibody
    • Serpin 2A antibody
    • Serpin A3G antibody
    • Serpina3g antibody
    • SPA3G_MOUSE antibody
    see all

Anti-Spi2A antibody [MoFo 29.2] images

  • Immunofluorescent analysis of Spi2A (green) showing staining in the cytoplasm of mouse kidney tissue (right) compared to a negative control without primary antibody (left). Formalin-fixed tissue was permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Tissue was probed with ab36772 in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4°C in a humidified chamber. Tissue was washed with PBST and incubated with a DyLight®-conjugated secondary antibody in PBS at room temperature in the dark. Nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x.

  • Immunofluorescent analysis of Spi2A (green) showing staining in the cytoplasm and nucleus of Jurkat cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab36772 in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight®-conjugated secondary antibody in PBS at room temperature in the dark. Nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x.

References for Anti-Spi2A antibody [MoFo 29.2] (ab36772)

This product has been referenced in:
  • Mizukami T  et al. Identification of transcripts commonly expressed in both hematopoietic and germ-line stem cells. Stem Cells Dev 17:67-80 (2008). IHC-P ; Mouse . Read more (PubMed: 18240954) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry. This product ab36772 was created by immunizing Armenian hamsters with a synthetic peptide conjugated to KLH. The resulting splenocytes were then fused to the tumor cell line SP2/0. We recommend using any HRP conjugat...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"