Overview

  • Product nameAnti-Spi2A antibody [MoFo29.2]
    See all Spi2A primary antibodies
  • Description
    Armenian Hamster monoclonal [MoFo29.2] to Spi2A
  • Specificityab28358 recognises mouse Serine Protease Inhibitor 2A (Spi2A).
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Mouse Spi2A aa 406-426 (C terminal).
    Sequence:

    NPERSTNFPNGEGASSQRC

  • Epitopeab28358 reacts with an epitope located in the region encoded by amino acids 406 - 426 including the C terminus of Spi2A.

Properties

Applications

Our Abpromise guarantee covers the use of ab28358 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 49 kDa.

Target

  • FunctionSerine and cysteine protease inhibitor. Can inhibit lysosomal papain-like proteases including the cathepsins B, G, H, K, L and V. Ineffective against elastase, granzyme A, granzyme B, or caspases 3, 8 or 9. Inhibition of cytoplasmic cathepsin B following release from the lysosome may protect cells from apoptosis. This may facilitate the survival of progenitor T-cells and the subsequent development of long term memory CD8 T-cells.
  • Tissue specificityExpressed in bone marrow (particularly hematopoietic stem cells), heart, kidney, liver, lung, skeletal muscle, spleen, testis, thymus and T-cells.
  • Sequence similaritiesBelongs to the serpin family.
  • Developmental stageT-cell specific expression rises during the differentiation of CD8 T-cell progenitors into memory CD8 T-cells.
  • DomainThe reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the serpin reactive site and the protease. The resulting inactive serpin-protease complex is highly stable (By similarity). Variability within the reactive center loop (RCL) sequences of Serpina3 paralogs may determine target protease specificity.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Serine protease inhibitor 2A antibody
    • Serine protease inhibitor A3G antibody
    • Serpin 2A antibody
    • Serpin A3G antibody
    • Serpina3g antibody
    • SPA3G_MOUSE antibody
    see all

Anti-Spi2A antibody [MoFo29.2] images

  • All lanes : Anti-Spi2A antibody [MoFo29.2] (ab28358)

    Lane 1 : NIH 3T3 transfected cells with Bcl3
    Lane 2 : NIH 3T3 transfected cells with Spi2A


    Predicted band size : 49 kDa

References for Anti-Spi2A antibody [MoFo29.2] (ab28358)

ab28358 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"