Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab42436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.25 µg/ml. Predicted molecular weight: 29 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/62500.

Target

  • FunctionSequence specific transcriptional activator which binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. Promotes development of plasmacytoid dendritic cells (pDCs), also known as type 2 DC precursors (pre-DC2) or natural interferon (IFN)-producing cells. These cells have the capacity to produce large amounts of interferon and block viral replication. May be required for B-cell receptor (BCR) signaling, which is necessary for normal B-cell development and antigenic stimulation.
  • Tissue specificityExpressed in plasmacytoid dendritic cells (pDCs) and B-cells, not expressed in T-cells or granulocytes. May also be enriched in stem cell populations of the liver.
  • Sequence similaritiesBelongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
  • DomainThe protein contains a weakly acidic N-terminal transactivation domain (TAD) followed by a second TAD rich in proline, serine and threonine. Each of these domains may be required for transcriptional activation of a subset of target genes.
  • Cellular localizationCytoplasm and Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • SPI B antibody
    • Spi B transcription factor antibody
    • Spib antibody
    • SPIB_HUMAN antibody
    • Transcription factor Spi-B antibody
    see all

Anti-SPIB antibody images

  • All lanes : Anti-SPIB antibody (ab42436) at 0.25 µg/ml

    Lane 1 : MW marker
    Lane 2 : Daudi cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 29 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-SPIB antibody (ab42436) at 0.25 µg/ml

    Lane 1 : Marker
    Lane 2 : Zebrafish brain homogenate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 29 kDa
    Observed band size : 51 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes

References for Anti-SPIB antibody (ab42436)

This product has been referenced in:
  • Wilkinson AC  et al. RUNX1 is a key target in t(4;11) leukemias that contributes to gene activation through an AF4-MLL complex interaction. Cell Rep 3:116-27 (2013). WB ; Human . Read more (PubMed: 23352661) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"