Overview

  • Product name
    Anti-SPIN3 antibody
  • Description
    Rabbit polyclonal to SPIN3
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 31-161 of Human SPIN3 (Q5JUX0).

  • Positive control
    • Human bone marrow tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121115 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.

Target

Images

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli. Recommended concentration of ab121115 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121115 at 1/150 dilution staining SPIN3 in Paraffin-emebedded Human bone marrow tissue by Immunohistochemistry. Note: strong nuclear and cytoplasmic positivity in bone marrow poietic cells.

References

ab121115 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab121115.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up