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Rabbit polyclonal to SPIN4
antigen sequence: MSPPTVPPMG VDGVSAYLMK KRHTHRKQRR KPTFLTRRNI VG, corresponding to N terminal amino acids 1-42 of Human SPIN4.
- Human liver tissue; Human Liver lysate.
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/250 - 1/500.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Belongs to the SPIN/STSY family.
Information by UniProt
- FLJ44984 antibody
- RP11-93B10.1 antibody
- SPIN4 antibody
Immunocytochemistry/ Immunofluorescence - Anti-SPIN4 antibody (ab122870)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleoli and cytoplasm. Recommended concentration of ab122870 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPIN4 antibody (ab122870)
ab122870 at 1/50 dilution staining SPIN4 in paraffin-embedded Human liver tissue by Immunohistochemistry.
Western blot - Anti-SPIN4 antibody (ab122870)
All lanes : Anti-SPIN4 antibody (ab122870) at 1/250 dilution
Lane 1 : RT 4 cell lysate
Lane 2 : U 251 MG cell lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Developed using the ECL technique.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"