Overview

  • Product nameAnti-SPIN4 antibody
    See all SPIN4 primary antibodies
  • Description
    Rabbit polyclonal to SPIN4
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: MSPPTVPPMG VDGVSAYLMK KRHTHRKQRR KPTFLTRRNI VG, corresponding to N terminal amino acids 1-42 of Human SPIN4.

  • Positive control
    • Human liver tissue; Human Liver lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122870 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

Anti-SPIN4 antibody images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleoli and cytoplasm. Recommended concentration of ab122870 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122870 at 1/50 dilution staining SPIN4 in paraffin-embedded Human liver tissue by Immunohistochemistry.
  • All lanes : Anti-SPIN4 antibody (ab122870) at 1/250 dilution

    Lane 1 : RT 4 cell lysate
    Lane 2 : U 251 MG cell lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver tissue lysate
    Lane 5 : Human tonsil tissue lysate

    Developed using the ECL technique

References for Anti-SPIN4 antibody (ab122870)

ab122870 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"