Overview

  • Product nameAnti-spindlin 1 antibody
    See all spindlin 1 primary antibodies
  • Description
    Rabbit polyclonal to spindlin 1
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 179-228 (YQLLDDYKEG DLRIMPDSND SPPAEREPGE VVDSLVGKQV EYAKEDGSKR) of Human spindlin 1 (NP_006708).

  • Positive control
    • Human fetal brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab118784 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 0.2 - 1 µg/ml. Predicted molecular weight: 30 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Anti-spindlin 1 antibody images

  • Anti-spindlin 1 antibody (ab118784) at 1 µg/ml + Human fetal brain lysate at 10 µg

    Predicted band size : 30 kDa
  • ab118784 staining spindlin 1 in human MDA-MB-468 cells by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde, permeabilized using 0.1% Triton X-100, blocked with 1% BSA for 15 minutes at 37°C and then incubated with ab118784 at a 1/100 dilution for 1 hour at 37°C. The secondary used was an ATTO488 conjugated F(ab')2 fragment donkey anti-rabbit polyclonal used at a 1/50 dilution.

    See Abreview

References for Anti-spindlin 1 antibody (ab118784)

This product has been referenced in:
  • Ducroux A  et al. The Tudor domain protein Spindlin1 is involved in intrinsic antiviral defense against incoming hepatitis B Virus and herpes simplex virus type 1. PLoS Pathog 10:e1004343 (2014). WB ; Human . Read more (PubMed: 25211330) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (cell line: MDA-MB-468 Mammary gland breast adenoca)
Specification cell line: MDA-MB-468 Mammary gland breast adenoca
Fixative Paraformaldehyde
Permeabilization Yes - 0,1% Triton X-100
Blocking step BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Peter Zentis

Verified customer

Submitted Mar 19 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"