Overview

  • Product nameAnti-Spindly antibody
    See all Spindly primary antibodies
  • Description
    Rabbit polyclonal to Spindly
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 458-488 of Human CCDC99 (NP_060255.3).

  • Positive control
    • A549 cell line lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage bufferPreservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab116002 was purified through a Protein A column, followed by peptide affinity purification.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab116002 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 70 kDa.

Target

  • FunctionRequired for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex. Also required for correct spindle orientation. Does not appear to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment.
  • Sequence similaritiesBelongs to the Spindly family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Nucleus. Cytoplasm > cytoskeleton > spindle pole. Localizes to the nucleus in interphase and to the kinetochore in early prometaphase. Relocalizes to the mitotic spindle pole before metaphase and is subsequently lost from the spindle poles after chromosome congression is completed. Removal of this protein from the kinetochore requires the dynein/dynactin complex.
  • Information by UniProt
  • Database links
  • Alternative names
    • AA409762 antibody
    • Arsenite related gene 1 protein antibody
    • Arsenite-related gene 1 protein antibody
    • CCDC 99 antibody
    • Ccdc99 antibody
    • Coiled coil domain containing 99 antibody
    • Coiled-coil domain-containing protein 99 antibody
    • FLJ20364 antibody
    • FLJ40690 antibody
    • hSpindly antibody
    • MGC124977 antibody
    • Protein Spindly antibody
    • rhabdomyosarcoma antigen MU RMS 40.4A antibody
    • Rhabdomyosarcoma antigen MU-RMS-40.4A antibody
    • Rrhabdomyosarcoma antigen protein MU RMS 40.4A antibody
    • SPDL1 antibody
    • SPDLY_HUMAN antibody
    • Spindle apparatus coiled coil domain containing protein 1 antibody
    • Spindle apparatus coiled coil protein 1 antibody
    • Spindly antibody
    • Spindly homolog antibody
    see all

Anti-Spindly antibody images

  • Anti-Spindly antibody (ab116002) at 1/100 dilution + A549 cell line lysate at 35 µg

    Predicted band size : 70 kDa

References for Anti-Spindly antibody (ab116002)

ab116002 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"