• Product nameAnti-Spindly antibody
    See all Spindly primary antibodies
  • Description
    Mouse monoclonal to Spindly
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Recombinant fragment
    Predicted to work with: Human
  • Immunogen

    Recombinant full length Human CCDC99 protein (AAH12568, 1 a.a. ~ 606 a.a) with tag.

  • Positive control
    • Recombinant tagged full length human CCDC99.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG2b
  • Light chain typekappa
  • Research areas


Our Abpromise guarantee covers the use of ab76670 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 70 kDa.

    This antibody has only been tested in WB against the recombinant protein used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionRequired for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex. Also required for correct spindle orientation. Does not appear to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment.
    • Sequence similaritiesBelongs to the Spindly family.
    • Post-translational
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localizationCytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Nucleus. Cytoplasm > cytoskeleton > spindle pole. Localizes to the nucleus in interphase and to the kinetochore in early prometaphase. Relocalizes to the mitotic spindle pole before metaphase and is subsequently lost from the spindle poles after chromosome congression is completed. Removal of this protein from the kinetochore requires the dynein/dynactin complex.
    • Information by UniProt
    • Database links
    • Alternative names
      • AA409762 antibody
      • Arsenite related gene 1 protein antibody
      • Arsenite-related gene 1 protein antibody
      • CCDC 99 antibody
      • Ccdc99 antibody
      • Coiled coil domain containing 99 antibody
      • Coiled-coil domain-containing protein 99 antibody
      • FLJ20364 antibody
      • FLJ40690 antibody
      • hSpindly antibody
      • MGC124977 antibody
      • Protein Spindly antibody
      • rhabdomyosarcoma antigen MU RMS 40.4A antibody
      • Rhabdomyosarcoma antigen MU-RMS-40.4A antibody
      • Rrhabdomyosarcoma antigen protein MU RMS 40.4A antibody
      • SPDL1 antibody
      • SPDLY_HUMAN antibody
      • Spindle apparatus coiled coil domain containing protein 1 antibody
      • Spindle apparatus coiled coil protein 1 antibody
      • Spindly antibody
      • Spindly homolog antibody
      see all

    Anti-Spindly antibody images

    • Anti-Spindly antibody (ab76670) at 1 µg/ml + Recombinant tagged full length human CCDC99 at 0.2 µg

      Goat Anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

      Predicted band size : 70 kDa
      Observed band size : 93 kDa (why is the actual band size different from the predicted?)

    References for Anti-Spindly antibody (ab76670)

    ab76670 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab76670.
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