The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500.
When using a fluorescent probe the recommended dilution is 1/50 – 1/200
Serine protease inhibitor which exhibits anti-trypsin activity (PubMed:7142173). In the pancreas, protects against trypsin-catalyzed premature activation of zymogens. In the male reproductive tract, binds to sperm heads where it modulates sperm capacitance by inhibiting calcium uptake and nitrogen oxide (NO) production.
Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded rat pancreas tissue sections labeling SPINK1 with ab203579 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.